Creating novel proteins by combining design and selection
نویسندگان
چکیده
منابع مشابه
Creating novel proteins by combining design and selection.
We present the results of combining design and selection to remodel a protein-peptide binding interface, using the peptide PTIEEVD and the TPR1 module interaction as our test case. We initially used the program Rosetta to interrogate possible TPR1 sequences compatible with binding the peptide PTIEEVD. Based on these results, we screened a small library of TPR1 variants, using a split GFP fluore...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2010
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzq015